Quantcast

Setting up a cheese lab

As you may remember from my last post, I am starting a lab focused on the study of the microbial ecosystems on cheese rinds. As it turns out, doing microbiology research requires a lot of stuff. At least that is what I am realizing as I set up my new lab… There's the equipment: pipettmen (to transfer small volumes of liquids), tubes (to hold the liquids), racks (to hold the tubes), and machines like a vortex (to mix the liquids in the tubes), freezers (to store samples), incubators (to grow microbes), and microscopes (to look at those tiny microbes). Then there are the reagents that make the experiments we will do possible, things like: growth media (microbe food), cell-lysis chemicals (detergents that bust cells open so we can extract their DNA), and enzymes like DNA polymerase (a purified enzyme that makes copies of DNA so we can study it- the one we use comes from a microbe found at vents at the bottom of the ocean!).

So, what does all this have to do with cheese??? You'll have to wait until next time to find out..

wfertman's picture

I found a fantastic article

I found a fantastic article on culturing limburger "red-smear" bacteria at home, using mayo-jar tops and hairpins: http://www.disknet.com/indiana_biolab/b122.htm:

This is a nice starting project because the desired organism has a distinctive color and produces a distinctive odor on certain medium. Therefore, when you isolate the organism you can be pretty sure you have Brevibacterium linens which is safe enough to eat. However, do not eat it because you are a beginner may have made some mistake.

A very professorial qualifier, there at the end.

I'm curious how many of these

I'm curious how many of these things are available to the lay person for some kitchen science? I can get agar at the supermarket for plating microbes and my hands after too much coffee could be a good vortexer, but what about a good detergent or even DNA polymerase -- probably not available to the public.

Hi Minda- That's a really

Hi Minda- That's a really good question! There is some fun information online about doing biology experiments at home- many of which don't require more than standard home supplies.

Here is a link to instructions on how to extract DNA from anything that has it- like strawberries, cells from inside your cheek, or cheese! http://learn.genetics.utah.edu/content/labs/extraction/howto/

Extracting DNA from cells is one of the first things a molecular biologist learns how to do. I still remember the first time I did this in lab, the cells were from the bacterium Bacillus subtilis, it was so amazing to see that big glob of DNA precipitate out of solution at the end of the experiment! It look like snot, honestly. So it was a combination of grossed out and amazed- maybe why it stuck with me!

For more advanced experiments, sites like labx.com sell used lab equipment at a discount, and you can buy microscope to look at microbes at many places online.

rachel

Post new comment

The content of this field is kept private and will not be shown publicly.
  • Web page addresses and e-mail addresses turn into links automatically.
  • Allowed HTML tags: <a> <em> <strong> <cite> <code> <ul> <ol> <li> <dl> <dt> <dd> <p> <br>
  • Lines and paragraphs break automatically.

More information about formatting options